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P2Y4 antibody

Catalog Number:OM266942
OM266942
Discount Price
OM266942-100ul
1~2 Week
100ul
OM266942-50ul
50ul
OM266942-20ul
20ul
1~2 Week
Product Profile
Product Name P2Y4 antibody
Antibody Type Primary Antibodies
Product description Rabbit Polyclonal antibody to P2Y4has purinergic nucleotide receptor activity; displays strongest agonist potency for ATP, UTP, ITP; may play a role in regulation of hepatic bile secretion [RGD, Feb 2006]
Immunogen Peptide (C)HEES ISRWA DTHQD, corresponding to residues 337-350 of rat P2Y4 (Accession O35811). Intracellular, C-terminus.
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications FACSICC/IFIHCIPWB
FACS:1:200~1:500
ICC/IF:1:200~1:500
IHC:1:50~1:200
IP:1:100~1:500
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity HumanMouseRat
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Synonyms pyrimidinergic receptor P2Y
G-protein coupled
4
P2ry4
Target P2Y4
Application

Application

Anti-P2Y4_Receptor - Western blot analysis of rat brain membranes: 1. Anti-P2Y4 Receptor antibody, (1:300). 2. Anti-P2Y4 Receptor antibody, preincubated with the control peptide antigen.

Application

Anti-P2Y4_Receptor - Expression of P2Y4 receptor in rat cerebellum Immunohistochemical staining of rat cerebellum using Anti-P2Y4 Receptor antibody. A. P2Y4 receptor (red) appears in astrocytic processes (arrows). B. In contrast, P2Y4 receptor does not appear in Purkinje neuronal dendrites stained for calbindin D28k (green) in the same brain section. This demonstrates the restriction of P2Y4 receptor to astrocytic fibers.
Application Notes FACS:1:200~1:500
ICC/IF:1:200~1:500
IHC:1:50~1:200
IP:1:100~1:500
WB:1:500~1:2000
Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.
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