| Product Name | p35 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to p35p35 / nck5a, is a neuron-specific activator of Cdk 5. Treatment of cultured neurons with cyclohexamide induces the rapid disappearance of p35 accompanied by parallel inactivation of the kinase activity of Cdk 5. The disappearance of p35 is blocked with proteasome inhibitors, indicating the involvement of proteasome. The degradation of p35 is induced with okadaic acid in the presence of ATP in neuron extracts. The degradation of p35 by proteasome in cultured neurons is stimulated by okadaic acid in the absence of cyclohexamide. Cdk 5 and its neuron specific regulator p35 are essential for neuronal migration and for the laminar configuration of the cerebral cortex. p35 / Cdk 5 kinase concentrates at the leading edges of axonal growth cones and regulates neurite outgrowth in cortical neurons in culture. p35 / Cdk 5 kinase co-localizes with Rac in neuronal growth cones. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 192-209 of Human p35 . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 35 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRatBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | CDK5P35 CDK5R MGC33831 NCK5A p23 p25 p35 p35nck5a |
|---|---|
| Target | p35 |
| Cellular Localization | Nuclear |
| Positive Control | Rat brain extract |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 35 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
