Application
Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320] antibody at 1:200 dilution.Application
Western blot analysis of RFC p37 protein expression in (A) HepG2, (B) LNCAP, (C) MCF7), (D) and SKOV3 whole cell lysates usingApplication
Application| Product Name | p37 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody to human replication factor C ( RFC ) p37Human Replication Factor C (RFC) is a hetero-pentameric protein consisting of the p140, p40, p38, p37 and p36 subunits. RFC functions in the loading of the DNA polymerase delta processivity factor (PCNA, Proliferating Cell nuclear Antigen) onto a primed DNA template during DNA synthesis and repair. RFC may also participate in the DNA damage checkpoint response with a PCNA-like hetero-trimer consisting of Hus 1, Rad 1 and Rad 9. |
| Immunogen | Recombinant protein encoding the full-length p37 subunit ofhuman RFC purified from E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | 1320 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researhcer.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | A1 MGC27291 RFC37 |
|---|---|
| Target | p37 |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Nuclear |
Application
Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320] antibody at 1:200 dilution.
Application
Western blot analysis of RFC p37 protein expression in (A) HepG2, (B) LNCAP, (C) MCF7), (D) and SKOV3 whole cell lysates using
Application
Application| Positive Control | HepG2, LNCAP, MCF7, SKOV3 |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000. Optimal dilutions/concentrations should be determined by the researhcer.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
