Application
WB analysis of lysates from HEK293 cells treated with UV irradiation using p38 MAPK (phospho Thr180/Tyr182) antibody pre-incubated with (1) the peptide immunogen, (2) a generic phosphothreonine-containing peptide, (3) a generic phosphotyrosine-containing peptide, (4) the non-phosphorylated peptide corresponding to the phosphopeptide, (5) no peptide, (6) the phosphopeptide derived from the corresponding region of JNK1&2 and (7) the phosphopeptide derived from the corresponding region of ERK1&2.| Product Name | p38 MAPK (phospho Thr180/Tyr182) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p38 MAPK (phospho Thr180/Tyr182)p38 is a 38 kDa Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK) that is fully activated by dual phosphorylation on threonine 180 and tyrosine 182, within the activation loop. The JNK and p38 pathways function to modulate cell-cycle, apoptotic and transcriptional responses to stress. This antibody has been shown to recognize the endogenous, active forms of p38 alpha, beta and gamma/ERK6. |
| Immunogen | Synthetic peptide (Human) derived from the region of human p38 that contains threonine 180 and tyrosine 182. This region is conserved among many species including human, rat, mouse, dog, and carp. |
| Modification | p-Thr180/Tyr182 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| For WB: Use at a dilution of 1:1,000. Predicted molecular weight: 38 kDa. Can be blocked with GTX25313 PC12 whole cell lysate, + / - Sorbitol treatment or GTX25253 p38 phospho T180 / Y182 and non-phospho peptides (pair) Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | p38 (phospho T180/Y182) p38 (phospho Thr180/Tyr182) p38 (pT180/pY182) p38 (pThr180/pTyr182) p38 phospho T180/Y182 p38 phospho Thr180/Tyr182 Phospho p38 (T180/Y182) Phospho p38 (Thr180/Tyr182) Phospho p38 (pT180/pY182) Phospho p38 (pThr180/pTyr182) |
|---|---|
| Target | p38 MAPK (phospho Thr180/Tyr182) |
| Tissue Specificity | Reacts with p38 alpha, beta and gamma. |
Application
WB analysis of lysates from HEK293 cells treated with UV irradiation using p38 MAPK (phospho Thr180/Tyr182) antibody pre-incubated with (1) the peptide immunogen, (2) a generic phosphothreonine-containing peptide, (3) a generic phosphotyrosine-containing peptide, (4) the non-phosphorylated peptide corresponding to the phosphopeptide, (5) no peptide, (6) the phosphopeptide derived from the corresponding region of JNK1&2 and (7) the phosphopeptide derived from the corresponding region of ERK1&2.| Positive Control | HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:1,000. Predicted molecular weight: 38 kDa. Can be blocked with GTX25313 PC12 whole cell lysate, + / - Sorbitol treatment or GTX25253 p38 phospho T180 / Y182 and non-phospho peptides (pair) Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -80ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH7.3, containing 50%glycerol, 1.0 mg/ml BSA, and 0.05% sodium azide |
