Application
Immunoprecipitation of p40 protein from A431 whole cell extracts using 5 μg of p40 antibody [N3C3] . Western blot analysis was performed using p40 antibody [N3C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using p40 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using p40 antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | p40 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p40 (Rab9 effector protein with kelch motifs)Rab9 effector required for endosome to trans-Golgi network (TGN) transport. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p40. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686P1077 antibody RAB9P40 antibody bA65N13.1 antibody p40 antibody RABEPK antibody rab9 effector protein with kelch motifs antibody 40 kDa Rab9 effector protein antibody Rab9 effector p40 antibody |
|---|---|
| Target | p40 |
| Molecular Weight(MW) | 41 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Endosome membrane |
Application
Immunoprecipitation of p40 protein from A431 whole cell extracts using 5 μg of p40 antibody [N3C3] . Western blot analysis was performed using p40 antibody [N3C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using p40 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using p40 antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
