| Product Name | p53 (acetyl Lys373) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p53 (acetyl Lys373)p53 tumor suppressor gene mutations are one of the most common genetic alterations in human tumors, including lymphomas. Altering or inactivating p53 by mutation or reaction with oncogenes or viruses may cause depletion of p53, or conversion of p53 from a tumor suppressor gene to a transforming oncogene. The nuclear protein p53 is associated with cell transformation and proliferation, with definitive possibilities as a marker in malignant lymphoma progression. p53's role in the multistep process leading to cancer is still being investigated, with an emphasis on its relation and control over additional tumor suppressors. |
| Immunogen | Synthetic peptide - amino acids 367-379 of human p53 (SHLKSK[AcK]GQSTSR) |
| Modification | acetyl Lys373 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:1,000-1:5,000. Detects GST-p53 acetylated by recombinant p300, HAT domain in an in vitro acetyl transferase assay. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TRP53 p53 acetylated (Lys373) P53 LFS1 FLJ92943 p53 (acetyl K373) p53 (acetyl Lys373) p53 acetyl K373 p53 acetyl Lys373 Acetyl p53 (K373) Acetyl p53 (Lys373) |
|---|---|
| Target | p53 (acetyl Lys373) |
| Tissue Specificity | Detects GST-p53 acetylated by recombinant p300, HAT domain in an in vitro acetyl transferase assay. |
| Application Notes | For WB: Use at a dilution of 1:1,000-1:5,000. Detects GST-p53 acetylated by recombinant p300, HAT domain in an in vitro acetyl transferase assay. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage add sterile glycerol (40-50%) aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Whole antiserum containing 0.05% sodium azide |
