Application
Antibody used in WB on Mouse SP2/0 at 2.5 ug/ml.Application
Antibody used in WB on Mouse at 1:1000 (Lane 1: 40ug C57/B6 control mouse G.I. Lane 2: 40ug C57/B6 mouse G.I. treated with Irinotecan Lane 3: 40ug p53 KO HCT-116 cells Lane 4: 40ug C57/B6 mouse treated with 15 Gy ionizing radiation ).| Product Name | TRP53 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TRP53, bbl, bfy, bhy, p44, p53, Tp53 |
| Product description | Trp53 is a protein found in elevated levels in a great variety of transformed cells1) Rotter, V., et al., (1984) Mol. Cell. Biol. 4 (2), 383-385. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse TRP53. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| TRP53 antibody can be used for detection of TRP53 by ELISA at 1:312500. TRP53 antibody can be used for detection of TRP53 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | TRP53 |
|---|---|
| Alternative Names | TRP53 bbl bfy bhy p44 p53 Tp53 |
| Molecular Weight(MW) | 43 kDa |
Application
Antibody used in WB on Mouse SP2/0 at 2.5 ug/ml.
Application
Antibody used in WB on Mouse at 1:1000 (Lane 1: 40ug C57/B6 control mouse G.I. Lane 2: 40ug C57/B6 mouse G.I. treated with Irinotecan Lane 3: 40ug p53 KO HCT-116 cells Lane 4: 40ug C57/B6 mouse treated with 15 Gy ionizing radiation ).| Positive Control | 1) SP2/0 Cell Lysate |
|---|---|
| Application Notes | TRP53 antibody can be used for detection of TRP53 by ELISA at 1:312500. TRP53 antibody can be used for detection of TRP53 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store TRP53 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
