Application
Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k using p53 (phospho Ser392) antibody p53 phosphorylated at Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.| Product Name | p53 (phospho Ser392) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p53 (phospho Ser392)Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser392 conjugated to KLH |
| Modification | p-Ser392 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TRP53 TP53 p53 LFS1 p53 (phospho S392) p53 (phospho Ser392) p53 (pS392) p53 (pSer392) p53 phospho S392 p53 phospho Ser392 Phospho p53 (S392) Phospho p53 (Ser392) Phospho p53 (pS392) Phospho p53 (pSer392) |
|---|---|
| Target | p53 (phospho Ser392) |
| Molecular Weight(MW) | 53 (note) |
| Cellular Localization | Interaction with BANP promotes nuclear localization. |
Application
Western blot of rat brain nuclear fraction lysate showing specific immunolabeling of the ~53k using p53 (phospho Ser392) antibody p53 phosphorylated at Ser392 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho Ser392 p53 antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For long-term storage, aliquot and store at -20 C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol. |
