Application
Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys379) Antibody. The picture on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys379) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | p53 (acetyl Lys379) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p53 (acetyl Lys379)This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human p53 around the acetylated site of Lys379. |
| Modification | acetyl Lys379 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-P |
| IF: 1:100~1:500 : |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Cellular tumor antigen p53 antibody Tumor suppressor p53 antibody Phosphoprotein p53 antibody TP53 antibody p53 antibody BCC7 antibody LFS1 antibody TRP53 antibody p53 (acetyl K379) antibody p53 (acetyl Lys379) antibody p53 acetyl K379 |
|---|---|
| Target | p53 (acetyl Lys379) |
| Molecular Weight(MW) | 43 kDa (note) |
| Tissue Specificity | p53 (Acetyl-Lys379) Antibody detects endogenous levels of total p53 protein only when acetylated at lysine379. |
| Cellular Localization | Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks. |
Application
Immunofluorescence analysis of HeLa cells, using p53 (Acetyl-Lys379) Antibody. The picture on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 (Acetyl-Lys379) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IF: 1:100~1:500 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
