Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P53 (phospho-Ser20) antibody .Application
Western blot analysis of extracts from COS7 cells treated with UV. Left: Using P53 (phospho-Ser20) antibody . Right: Using the same antibody preincubated with synthesized peptide.| Product Name | p53 (phospho Ser20) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to P53 (phospho S20)This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq] |
| Modification | p-Ser20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseMonkey |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | LFS1 TRP53 p53 |
|---|---|
| Target | P53 (phospho S20) |
| Cellular Localization | Interaction with BANP promotes nuclear localization. |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P53 (phospho-Ser20) antibody .
Application
Western blot analysis of extracts from COS7 cells treated with UV. Left: Using P53 (phospho-Ser20) antibody . Right: Using the same antibody preincubated with synthesized peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
