Application
Untreated (–) and treated (+) zebrafish embryos extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Tp53 antibody detects Tp53 protein on UV-irradiated whole-mount zebrafish embryos (right panel) by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Tp53 antibody dilution: 1:200.| Product Name | p53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p53 (tumor protein p53)Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of bax and fas antigen expression, or by repression of Bcl-2 expression. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of zebrafish p53. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-W |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Zebra Fish |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | tumor suppressor homolog p53 antibody cellular tumor antigen p53 antibody etID22686.5 antibody tumor suppressor p53 antibody brp53 antibody drp53 antibody fb40d06 antibody p53 antibody wu:fb40d06 antibody zgc:111919 antibody tp53 antibody tumor |
|---|---|
| Target | p53 |
| Molecular Weight(MW) | 42 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) , Nucleus |
Application
Untreated (–) and treated (+) zebrafish embryos extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with p53 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Tp53 antibody detects Tp53 protein on UV-irradiated whole-mount zebrafish embryos (right panel) by immunohistochemical analysis. Sample: Paraformaldehyde-fixed zebrafish embryos. Tp53 antibody dilution: 1:200.| Positive Control | 3dpf zebrafish embryos treated with UVC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
