| Product Name | p53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3F301] to p53p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 3F301 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-PIPWB |
| FACS: Use at an assay dependent dilution. IHC-P: Use at a concentration of 2 - 4 μg/ml.Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. IP: Use at a concentration of 2 μg/mg lysate (GTX17869 will detect both native and denatured forms of the target, use protein G beads). WB: Use at a concentration of 0.5 - 1 μg/ml. Detects a band of approximately 53 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Cys51Stop TRP53 TP53 HGNC11998 p53 tumor suppressor tumor protein p53 |
|---|---|
| Target | TP53 |
| Cellular Localization | Cytoplasmic and Nuclear |
| Positive Control | SKBR-3 cells or Colon carcinoma |
|---|---|
| Application Notes | FACS: Use at an assay dependent dilution. IHC-P: Use at a concentration of 2 - 4 μg/ml.Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. IP: Use at a concentration of 2 μg/mg lysate (GTX17869 will detect both native and denatured forms of the target, use protein G beads). WB: Use at a concentration of 0.5 - 1 μg/ml. Detects a band of approximately 53 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
