Application
WB analysis of HEK293 cell lysate using p53 antibody [DO-7].Application
IHC-P analysis of human breast carcinoma tissue using p53 antibody [DO-7].| Product Name | p53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [DO-7] to p53This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons (PMIDs: 12032546, 20937277). [provided by RefSeq, Feb 2013] |
| Immunogen | Recombinant human p53. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | DO-7 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIHC-PIPWB |
| Western Blotting: Max Dilution 1/1000: |
|
| Species Reactivity | HumanBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BCC7 TRP53 P53 TP53 LFS1 tumor protein p53 |
|---|---|
| Target | p53 |
| Tissue Specificity | This antibody recognizes an epitope at the N-terminal end of p53 between amino acids 20-25, binding to both wild type and mutant forms. |
Application
WB analysis of HEK293 cell lysate using p53 antibody [DO-7].
Application
IHC-P analysis of human breast carcinoma tissue using p53 antibody [DO-7].| Application Notes | Western Blotting: Max Dilution 1/1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
