Application
Human Lung Adenocarcinoma stained with anti-p53 antibodyApplication
Human Colon Adenocarcinoma stained with anti-p53 antibodyApplication
Human Colon Carcinoma stained with anti-p53 antibodyApplication
Human Stomach Adenocarcinoma stained with anti-p53 antibodyApplication
Human Ovarian Adenocarcinoma stained with anti-p53 antibodyApplication
Human Bladder Transitional Cell Carcinoma stained with anti-p53 antibodyApplication
Human Hepatocellular Cell Carcinoma stained with anti-p53 antibodyApplication
Human Cervical Squamous Cell Carcinoma stained with anti-p53 antibodyApplication
Human Breast Ductal Cell Carcinoma stained with anti-p53 antibodyApplication
Human B Cell Lymphoma stained with anti-p53 antibody| Product Name | p53 antibody [SP5] (ready-to-use) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [SP5] to p53This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity. [provided by RefSeq] |
| Immunogen | Recombinant human full-length wild type p53 protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | SP5 |
| Host Species | Rabbit |
| Tested Applications | IHC,IHC-P |
| We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | P53, TRP53, TP53, LFS1, FLJ92943 |
|---|---|
| Target | p53 |
| Tissue Specificity | This antibody recognizes p53 protein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to mdm2, SV40 T antigen and human papilloma virus E6 protein p53 senses DNA damage and possibly facilitating repair. Mutation involving p53 is found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.This antibody crossreacts with Human. |
| Cellular Localization | Nuclear |
Application
Human Lung Adenocarcinoma stained with anti-p53 antibody
Application
Human Colon Adenocarcinoma stained with anti-p53 antibody
Application
Human Colon Carcinoma stained with anti-p53 antibody
Application
Human Stomach Adenocarcinoma stained with anti-p53 antibody
Application
Human Ovarian Adenocarcinoma stained with anti-p53 antibody
Application
Human Bladder Transitional Cell Carcinoma stained with anti-p53 antibody
Application
Human Hepatocellular Cell Carcinoma stained with anti-p53 antibody
Application
Human Cervical Squamous Cell Carcinoma stained with anti-p53 antibody
Application
Human Breast Ductal Cell Carcinoma stained with anti-p53 antibody
Application
Human B Cell Lymphoma stained with anti-p53 antibody| Positive Control | Colon carcinoma |
|---|---|
| Application Notes | We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
