Application
Western blot analysis using Manti-p53 cln. X77 antibody at 10 µg/ml on HCT116 cell lysate (1), HCT116 cell lysate activated with adriamycin (2), p21-/- cell lysate (3), P21-/- cell lysate activated with ADR (4) and p53-/- activated with ADR. ADR activates p53 in cells. Also shown is a graphic representation of the epitope location.| Product Name | p53 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [X77] to p53p53 plays a major role in the cellular response to DNA damage and other genomic aberrations. The activation of p53 can lead to either cell cycle arrest and DNA repair, or apoptosis. p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro. |
| Immunogen | Full length protein (Xenopus laevis). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | X77 |
| Host Species | Mouse |
| Tested Applications | ELISAIPWB |
| ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 10 μg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseXenopus |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Gene Synonyms | Cys51Stop TRP53 TP53 HGNC11998 p53 tumor suppressor tumor protein p53 |
|---|---|
| Target | TP53 |
| Cellular Localization | Cytoplasmic and Nuclear |
Application
Western blot analysis using Manti-p53 cln. X77 antibody at 10 µg/ml on HCT116 cell lysate (1), HCT116 cell lysate activated with adriamycin (2), p21-/- cell lysate (3), P21-/- cell lysate activated with ADR (4) and p53-/- activated with ADR. ADR activates p53 in cells. Also shown is a graphic representation of the epitope location.| Application Notes | ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 - 10 μg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.08% Sodium Azide. Constituents: PBS |
