Rabbit polyclonal to p57 Kip2p57 / KIP 2 protein is a inhibitor of several G1 cyclin complexes and is a negative regulator of cell proliferation. The gene encoding human p57 / KIP 2 protein is located on chromosome 11p15.5, a region implicated in both sporadic cancers, Wilm's tumor and Beckwith-Wiedemann syndrome.
Immunogen
Synthetic peptide (Human).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IHCIHC-FIHC-P
Immunohistochemistry: use at a dilution of 1/25 - 1/50 for 30mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.:
Species Reactivity
HumanMouseBovine
Concentration
1mg/ml
Target Information
Gene Synonyms
BWCR BWS KIP2 WBS p57
Target
p57 Kip2
Tissue Specificity
This antibody reacts with a 57 kDa protein.
Cellular Localization
Nuclear.
Application
Application
IHC-P analysis of human placenta using p57 Kip2 antibody.
Application
IHC Image using - Detection of p57 Kip2 by IHC in Human Colon Carcinoma
Positive Control
Colon Carcinoma
Application Notes
Immunohistochemistry: use at a dilution of 1/25 - 1/50 for 30mins at RT (ABC method). Optimal dilutions / concentrations should be determined by the end user. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval using 10mM citrate buffer pH 6.0 before commencing with the IHC staining protocol.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.