Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p73 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using p73 antibody at 1:100 dilution.Application
p73 antibody [N1C1] detects p73 protein at nucleus in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. p73 antibody [N1C1] diluted at 1:250.Application
p73 antibody [N1C1] detects p73 protein at nucleus in human SCC xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human SCC xenograft . p73 antibody [N1C1] diluted at 1:250.| Product Name | p73 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p73 (tumor protein p73)This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p73. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rat, ZebrafishRat (94%), Zebrafish (80%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P73 antibody TP73 antibody p53-like transcription factor antibody p53-related protein antibody tumor protein p73 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p73 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Jurkat 7.5% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using p73 antibody at 1:100 dilution.
Application
p73 antibody [N1C1] detects p73 protein at nucleus in human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. p73 antibody [N1C1] diluted at 1:250.
Application
p73 antibody [N1C1] detects p73 protein at nucleus in human SCC xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human SCC xenograft . p73 antibody [N1C1] diluted at 1:250.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
