Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein at cytoplasm by immunofluorescent analysis. Sample: Culture rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG 7.5% SDS PAGE diluted at 1:2000Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor antibody at a dilution of 1:2000.Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p75 NGF Receptor antibody diluted at 1:500.IF
(A) Representative immunofluorescence micrographs showing the expression levels of Schwann cell markers (S100, GFAP, P75NGRF) in twelve experimental groups (BMSCs, BMSCs + ODM, BMSCs + RSC96-exos, BMSCs + Fb-exos, BMSCs + NSC-exos, BMSCs + ODM + RSC96-exos, BMSCs + ODM + Fb-exos, BMSCs + ODM + NSC-exos, induced BMSCs + RSC96-exos, induced BMSCs + Fb-exo, induced BMSCs + NSC-exo and RSC96) (magnification, x100). Scale bar, 50 μm.WB
(B) Representative western blotting of the protein expression levels of S100, GFAP, and P75NGRF in the twelve groupsWB
(F) Representative immunoblot of protein expression levels of S100, GFAP, NGRF, Sox10, EGR2 and GAPDH in control BMSCs, induced BMSCs, BMSCs + RSC96 exo, BMSCs + Fb exo or RSC96 cells.| Product Name | p75 NGF Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to p75 NGF Receptor (nerve growth factor receptor (TNFR superfamily, member 16))Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human p75 NGF Receptor. The exact sequence is proprietary. |
| Modification | Acetylated |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:1000 IHC-P:1:50~1:100 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, BovineMouse (92%), Bovine (97%) |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | CD271 antibody Gp80-LNGFR antibody TNFRSF16 antibody p75(NTR) antibody p75NTR antibody NGFR antibody tumor necrosis factor receptor superfamily member 16 antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein at cytoplasm by immunofluorescent analysis. Sample: Culture rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:250. Blue: Fluoroshield with DAPI .
Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: p75 NGF Receptor protein stained by p75 NGF Receptor antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG 7.5% SDS PAGE diluted at 1:2000
Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with p75 NGF Receptor antibody at a dilution of 1:2000.
Application
p75 NGF Receptor antibody detects p75 NGF Receptor protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. p75 NGF Receptor antibody diluted at 1:500.
IF
(A) Representative immunofluorescence micrographs showing the expression levels of Schwann cell markers (S100, GFAP, P75NGRF) in twelve experimental groups (BMSCs, BMSCs + ODM, BMSCs + RSC96-exos, BMSCs + Fb-exos, BMSCs + NSC-exos, BMSCs + ODM + RSC96-exos, BMSCs + ODM + Fb-exos, BMSCs + ODM + NSC-exos, induced BMSCs + RSC96-exos, induced BMSCs + Fb-exo, induced BMSCs + NSC-exo and RSC96) (magnification, x100). Scale bar, 50 μm.
WB
(B) Representative western blotting of the protein expression levels of S100, GFAP, and P75NGRF in the twelve groups
WB
(F) Representative immunoblot of protein expression levels of S100, GFAP, NGRF, Sox10, EGR2 and GAPDH in control BMSCs, induced BMSCs, BMSCs + RSC96 exo, BMSCs + Fb exo or RSC96 cells.| Positive Control | NT2D1 , PC-3 , U87-MG , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.: WB:1:500~1:1000 IHC-P:1:50~1:100 ICC/IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
