Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using p90RSK(Phospho-Thr348)Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using p90RSK (Phospho-Thr348) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells using p90RSK (Phospho-Thr348) Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK (Phospho-Thr348) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | p90 RSK1 (phospho Thr348) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to p90 RSK1 (phospho Thr348)Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB. |
| Immunogen | Peptide sequence around phosphorylation site of threonine 348 (S-R-T(p)-P-R) derived from Human p90RSK. |
| Modification | p-Thr348 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HU-1, MAPKAPK1A, RSK, RSK1, RPS6KA1, MAPK-activated protein kinase 1a, MAPKAP kinase 1a, p90-RSK 1, ribosomal S6 kinase 1, ribosomal protein S6 kinase alpha 1, dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1), MAPKAPK-1a, RSK-1, 90 kDa riboso |
|---|---|
| Target | p90 RSK1 (phospho Thr348) |
| Molecular Weight(MW) | 90 (note) |
Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using p90RSK(Phospho-Thr348)
Application
Western blot analysis of extracts from Hela cells untreated or treated with UV using p90RSK (Phospho-Thr348) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells using p90RSK (Phospho-Thr348) Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90RSK (Phospho-Thr348) Antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
