Application
PABPC4 antibody detects PABPC4 protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. PABPC4 antibody dilution: 1:500.Application
PABPC4 antibody detects PABPC4 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PABPC4 antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000| Product Name | PABPC4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PABPC4 (poly(A) binding protein, cytoplasmic 4 (inducible form))Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PABPC4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, BovineRat (96%), Zebrafish (90%), Xenopus laevis (91%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | APP-1 antibody, APP1 antibody, FLJ43938 antibody, PABP4 antibody, iPABP antibody, PABPC4 antibody, PABP-4 antibody, polyadenylate-binding protein 4 antibody, inducible poly(A)-binding protein antibody, activated-platelet protein 1 antibody, poly(A)-binding |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
PABPC4 antibody detects PABPC4 protein at cytosol on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. PABPC4 antibody dilution: 1:500.
Application
PABPC4 antibody detects PABPC4 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PABPC4 antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
