Application
WB analysis of HepG2 cells using PABPC4 antibody at 0.2-1μg/ml.| Product Name | PABPC4 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PABPC4Poly(A)-binding proteins (PABPs) bind to the poly(A) tail present at the 3-prime ends of most eukaryotic mRNAs. PABPC4 or IPABP (inducible PABP) was isolated as an activation-induced T-cell mRNA encoding a protein. Activation of T cells increased PABPC4 mRNA levels in T cells approximately 5-fold. PABPC4 contains 4 RNA-binding domains and proline-rich C terminus. PABPC4 is localized primarily to the cytoplasm. It is suggested that PABPC4 might be necessary for regulation of stability of labile mRNA species in activated T cells. PABPC4 was also identified as an antigen, APP1 (activated-platelet protein-1), expressed on thrombin-activated rabbit platelets. PABPC4 may also be involved in the regulation of protein translation in platelets and megakaryocytes or may participate in the binding or stabilization of polyadenylates in platelet dense granules. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008] |
| Immunogen | A synthetic peptide corresponding to an Internal region of Human PABPC4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Cow: 93%; Dog: 92%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100% |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PABP4 APP1 PABPC4 IPABP |
|---|---|
| Target | PABPC4 |
| Molecular Weight(MW) | 71 KDa (note) |
Application
WB analysis of HepG2 cells using PABPC4 antibody at 0.2-1μg/ml.| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
