Application
Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255) . Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.| Product Name | PAG antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 255] to PAGCsk binding protein, also known as PAG (phosphoprotein associated with GEMs), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck. |
| Immunogen | Recombinant intracellular fragment, corresponding to amino acids 97-432 of Human CSK Binding Protein. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-255 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| WB: Use at an assay dependant dilution. Detects a band of approximately 80 kDa (predicted molecular weight: 46 kDa). Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CBP FLJ37858 MGC138364 PAG |
|---|---|
| Target | PAG |
| Tissue Specificity | The antibody recognizes an epitope (aa 235-280) located in the cytoplasmic domain of Csk-binding protein (Cbp). |
Application
Immunohistochemistry staining (paraffin-embedded sections) using anti-Cbp/PAG (MEM-255) . Cbp/PAG is expressed in germinal centers of lymph node lymphoid follicle and in follicular lymphoma (it is absent from mantle zone). Cbp/PAG is also expressed more weakly in T cells in tonsil and the thymic medulla.| Positive Control | RAMOS and Jurkat cells |
|---|---|
| Application Notes | WB: Use at an assay dependant dilution. Detects a band of approximately 80 kDa (predicted molecular weight: 46 kDa). Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
