Application
Immunofluorescence analysis of methanol-fixed HeLa, using PAI3 antibody at 1:200 dilution.Application
PAI3 antibody detects SERPINA5 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. PAI3 antibody dilution: 1:500.Application
PAI3 antibody detects PAI3 protein by western blot analysis. HepG2 whole cell extracts (30 μg) and conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with PAI3 antibody diluted at 1:10000.| Product Name | PAI3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAI3 (serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAI3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, ChimpanzeeRhesus Monkey (92%), Chimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PAI3 antibody PCI antibody PLANH3 antibody PROCI antibody SERPINA5 antibody acrosomal serine protease inhibitor antibody plasma serine protease inhibitor antibody serpin A5 antibody plasminogen activator inhibitor-3 antibody serine (or cysteine) p |
|---|---|
| Molecular Weight(MW) | 46 kDa (note) (2) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PAI3 antibody at 1:200 dilution.
Application
PAI3 antibody detects SERPINA5 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. PAI3 antibody dilution: 1:500.
Application
PAI3 antibody detects PAI3 protein by western blot analysis. HepG2 whole cell extracts (30 μg) and conditioned medium were separated by 10% SDS-PAGE, and the membrane was blotted with PAI3 antibody diluted at 1:10000.| Positive Control | HepG2 conditioned medium |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
