Application
Sample (30 µg of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PAK1 antibody [N2C2], Internal detects PAK1 protein at on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. PAK1 antibody [N2C2], Internal dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: BCL-1 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of methanol-fixed A431, using PAK1 antibody at 1:500 dilution.Application
PAK1 antibody [N2C2], Internal detects PAK1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE PAK1 antibody [N2C2], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PAK1 antibody [N2C2], Internal detects PAK1 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. PAK1 antibody [N2C2], Internal dilution: 1:500.| Product Name | PAK1 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAK1 (p21 protein (Cdc42/Rac)-activated kinase 1)PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, BovineZebrafish (84%), Xenopus laevis (84%), Chicken (95%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC130000 antibody MGC130001 antibody PAKalpha antibody PAK1 antibody p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog yeast) antibody alpha-PAK antibody p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related) antibody p65-PAK antibody serine/thre |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Cell junction , focal adhesion |
Application
Sample (30 µg of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PAK1 antibody [N2C2], Internal detects PAK1 protein at on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. PAK1 antibody [N2C2], Internal dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: BCL-1 7.5% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of methanol-fixed A431, using PAK1 antibody at 1:500 dilution.
Application
PAK1 antibody [N2C2], Internal detects PAK1 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE PAK1 antibody [N2C2], Internal dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PAK1 antibody [N2C2], Internal detects PAK1 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. PAK1 antibody [N2C2], Internal dilution: 1:500.| Positive Control | A431 , BCL-1 , PC-12 , SH-SY5Y |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
