Application
Western blot analysis of PAK3 (pS154) expression in HEK293T EGF-treated (A); NIH3T3 (B); mouse brain (C); SP2/0 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.| Product Name | PAK3 (phospho Ser154) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAK3 (phospho Ser154)PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PAK3. The exact sequence is proprietary. |
| Modification | p-Ser154 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | bPAK beta-PAK PAK3beta PAK-3 MRX47 MRX30 p21 protein (Cdc42/Rac)-activated kinase 3 OPHN3 PAK3 |
|---|---|
| Target | PAK3 (phospho Ser154) |
| Tissue Specificity | Recognizes endogenous levels of PAK3 (pS154) protein. |
Application
Western blot analysis of PAK3 (pS154) expression in HEK293T EGF-treated (A); NIH3T3 (B); mouse brain (C); SP2/0 EGF-treated (D); H9C2 EGF-treated (E) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
