ICC/IF
Immunofluorescence analysis of Hela cells using PAK3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.WB
Western blot analysis using PAK3 antibody against K562(1),Hepg2(2),Nih3t3(3) cell,Rat testes tissue(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Product Name | Anti-PAK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:100-1:500 WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Antigen peptide affinity chromatography |
| Gene Symbol | PAK3 |
|---|---|
| Gene Synonyms | 5330420P17Rik A130002K10Rik alpha-PAK ARA beta-PAK bPAK D16Ertd269e gamma-PAK IDDMSSD KNO2 mKIAA4182 MRX30 MRX47 OPHN3 p65-PAK PAK-1 PAK-2 PAK-3 PAK3beta PAK65 Pak65alpha Pak65beta Paka PAKalpha PAKgamma Stk4 XLID30 |
| Gene Full Name | p21 (RAC1) activated kinase 3 |
| Gene Summary | The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of a non-syndromic form of X-linked intellectual disability. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2017] |
| Alternative Names | activated protein kinase alpha ADRB2 adriamycin resistance-associated alpha-PAK aPAK beta-PAK BPAK CDC42/RAC effector kinase PAK-A CDC42/RAC effector kinase PAK-B CDKN1A CLAP EC 2.7.11.1 gamma pak gamma-PAK hPAK3 kinase PAK1 oligophrenin-3 p21 (CDKN1A)-activated kinase 1 p21 (CDKN1A)-activated kinase 2 p21 (CDKN1A)-activated kinase 3 P21 CDKN1A-activated kinase 1 p21 protein (Cdc42/Rac)-activated kinase 1 p21 protein (Cdc42/Rac)-activated kinase 2 p21 protein (Cdc42/Rac)-activated kinase 3 p21-activated kinase 1 p21-activated kinase 2 p21-activated kinase 3 p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog yeast) p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related) p21/Cdc42/Rac1-activated kinase 3 (yeast Ste20-related) p21cdc42/rac1-activated serine/threonine kinase p58 p65-PAK p68-PAK PAK 1 PAK alpha PAK beta PAK gamma PAK-1 PAK-2 PAK-3 PAK-a PAK-B PAK-y protein kinase protein kinase MUK2 S6/H4 kinase serine/threonine-protein kinase PAK 2-like STE20 homolog yeast yPAK |
| Molecular Weight(MW) | 62kDa(observed：58kDa) |
| Cellular Localization | Cytoplasm |
| SwissProt ID | O75914 |
|---|
ICC/IF
Immunofluorescence analysis of Hela cells using PAK3 antibody (green). Blue: DAPI fluorescent DNA dye. Red: Actin filaments have been labeled with Omnimabs® 594-Phalloidin.Cells are fixed in 4% paraformaldehyde at room temperature for 15 minutes. Then, they are permeabilized with a PBS (OM750003) solution containing 0.1% Triton X-100(OM750021) at room temperature for 15 minutes. Subsequently, the cells are blocked with 10% non - immune goat serum(OM760028) at room temperature for 1 hour.The cells are incubated overnight at 4°C with the primary antibody diluted 1:100 in PBS. The secondary antibody, Omnimabs® 488 Goat Ant-Rabbit IgG(H&L) (Green,OM643486), is diluted at a ratio of 1:400 and incubated with the cells for 1 hour.Nuclear DNA is labeled with DAPI (Blue,OM643160). F-actin is stained with Omnimabs® 594-Phalloidin (Red,OM750007) diluted 1:100 for 30 minutes.
WB
Western blot analysis using PAK3 antibody against K562(1),Hepg2(2),Nih3t3(3) cell,Rat testes tissue(4) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:15000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.
ELISA
Coat the plate with immunizing antigen.Blue line： Immune serum; Orange line: Pre-immune serum.| Application Notes | ICC/IF:1:100-1:500 WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
