Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE diluted at 1:1000| Product Name | PAK3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAK3 (serine/threonine-protein kinase PAK 3)PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. The protein encoded by this gene forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAK3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chimpanzee, BovineRat (100%), Xenopus laevis (99%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDKN1A antibody MRX30 antibody MRX47 antibody OPHN3 antibody PAK3beta antibody bPAK antibody hPAK3 antibody PAK3 antibody p21 (CDKN1A)-activated kinase 3 antibody oligophrenin-3 antibody p21-activated kinase 3 antibody serine/threonine-protein k |
|---|---|
| Molecular Weight(MW) | 62 kDa (note) (16) |
Application
Sample (30 ug of whole cell lysate) A: IMR32 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: BCL-1 7.5% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 , NIH-3T3 , JC |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
