Application
Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 ug/mL.Application
Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 ug/mL.Application
Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 ug/mL.| Product Name | PAK4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAK4PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 interacts specifically with the GTP-bound form of Cdc42Hs and weakly activates the JNK family of MAP kinases. PAK4 is a mediator of filopodia formation and may play a role in the reorganization of the actin cytoskeleton. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Immunogen | PAK4 antibody was raised against a 13 amino acid synthetic peptide from near the center of human PAK4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| WB: 1 - 2 ug/ml. IHC-P: 10 ug/ml. IF: 20 ug/ml: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | p21 protein (Cdc42/Rac)-activated kinase 4 |
|---|---|
| Target | PAK4 |
| Tissue Specificity | PAK4 antibody is predicted to not cross-react with other PAK family proteins. |
Application
Immunohistochemistry of PAK4 in human colon tissue with PAK4 antibody at 10 ug/mL.
Application
Immunofluorescence of PAK4 in Human Colon tissue with PAK4 antibody at 20 ug/mL.
Application
Western blot analysis of PAK4 in SW480 lysate with PAK4 antibody at (A) 1 and (B) 2 ug/mL.| Application Notes | WB: 1 - 2 ug/ml. IHC-P: 10 ug/ml. IF: 20 ug/ml: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Be stored at 4oC for three months and -20oC, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PBS containing 0.02% sodium azide. |
