Application
PAK6 antibody immunoprecipitates PAK6 protein in IP experiments. IP samples: PC-3 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of PAK6 protein by 4 μg PAK6 antibody 7.5 % SDS-PAGE The immunoprecipitated PAK6 protein was detected by PAK6 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PAK6 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: PAK6 transfected 293T lysates 7.5% SDS PAGE diluted at 1:5000| Product Name | PAK6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAK6 (serine/threonine-protein kinase PAK 6)This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAK6. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (97%), Rat (98%), Rhesus Monkey (99%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PAK5 antibody PAK6 antibody PAK-5 antibody PAK-6 antibody p21(CDKN1A)-activated kinase 6 antibody serine/threonine-protein kinase PAK 6 antibody p21-activated protein kinase 6 antibody p21-activated kinase 6 antibody p21 protein (Cdc42/Rac)-activat |
|---|---|
| Molecular Weight(MW) | 75 kDa (note) (4) |
Application
PAK6 antibody immunoprecipitates PAK6 protein in IP experiments. IP samples: PC-3 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of PAK6 protein by 4 μg PAK6 antibody 7.5 % SDS-PAGE The immunoprecipitated PAK6 protein was detected by PAK6 antibody diluted at 1:500. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using PAK6 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: PAK6 transfected 293T lysates 7.5% SDS PAGE diluted at 1:5000| Positive Control | 293T transfected recombinant PAK6 protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
