Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody diluted at 1:4000.Application
pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in ice-cold MeOH for 5 min. Green: pan Cadherin protein stained by pan Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody diluted at 1:5000.| Product Name | pan Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to pan CadherinThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-termius region of N-Cadherin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Chicken, BovineMouse (100%), Rat (100%), Dog (100%), Chicken (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | pan Cadherin |
|---|---|
| Molecular Weight(MW) | 100 kDa (note) (2) |
| Cellular Localization | Cell membrane |
Application
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody diluted at 1:4000.
Application
pan Cadherin antibody detects pan Cadherin protein at cell membrane by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in ice-cold MeOH for 5 min. Green: pan Cadherin protein stained by pan Cadherin antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with pan Cadherin antibody diluted at 1:5000.| Positive Control | A431 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
