Application
IHC Image using - Detection of Pan Cadherin by IHC in Human Squamous cell Carcinoma| Product Name | Pan Cadherin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Polyclonal Antibody to Pan CadherinThis antibody reacts with 120 kD protein. Cadherins are calcium dependent cell adhesion molecules, which play important role in the growth and development of cells. In adhesion junctions, cadherins are bound to b and g catenins, which in turn bind to a catenin. Cadherins play important role in tumor invasion and metastasis. This antibody crossreacts with mouse, rat, chicken, dog, monkey and xenopus. |
| Immunogen | A synthetic peptide from the c-terminus of chicken N-cadherin. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Chicken |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Pan Cadherin |
|---|---|
| Cellular Localization | Cell membrane |
Application
IHC Image using - Detection of Pan Cadherin by IHC in Human Squamous cell Carcinoma| Positive Control | Tonsil |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
