Application
ChIP was performed with NIH3T3 extracts and 4 μg of pan-HP1 antibody. The precipitated DNA was detected by PCR with primers targeting to the rDNA promoter and a subtelomeric sequence of chromosome 19.Application
WB analysis of nuclear extracts from HeLa cells (20 μg) using pan-HP1 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ICC/IF analysis of HeLa cells using pan-HP1 antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with pan-HP antibody at a dilution of 1:500.| Product Name | pan-HP1 antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HP1 alpha, HP1 beta and HP1 gammaThis gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | Polyclonal antibody raised in rabbit against human HP1 β (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWBChIP |
| Western blotting (1:1,000): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | chromobox homolog 5 CBX5 HP1A HP1 |
|---|---|
| Target | HP1 alpha/HP1 beta/HP1 gamma |
Application
ChIP was performed with NIH3T3 extracts and 4 μg of pan-HP1 antibody. The precipitated DNA was detected by PCR with primers targeting to the rDNA promoter and a subtelomeric sequence of chromosome 19.
Application
WB analysis of nuclear extracts from HeLa cells (20 μg) using pan-HP1 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ICC/IF analysis of HeLa cells using pan-HP1 antibody (green) and DAPI (blue). Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with pan-HP antibody at a dilution of 1:500.| Application Notes | Western blotting (1:1,000): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
