Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PANK1 antibody at 1:500 dilution.Application
Immunofluorescence analysis of methanol-fixed Hep G2, using PANK1 antibody at 1:500 dilution.Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:5000| Product Name | PANK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PANK1 (pantothenate kinase 1)This gene encodes a protein belonging to the pantothenate kinase family. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by CoA. Alternative splicing has been observed at this locus and three variants, each encoding a distinct isoform, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PANK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC24596 antibody PANK antibody PANK1a antibody PANK1b antibody PANK1 antibody pantothenic acid kinase 1 antibody pantothenate kinase 1 antibody |
|---|---|
| Molecular Weight(MW) | 64 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (50 ug of whole cell lysate) A: mouse liver 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PANK1 antibody at 1:500 dilution.
Application
Immunofluorescence analysis of methanol-fixed Hep G2, using PANK1 antibody at 1:500 dilution.
Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:5000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
