Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500Application
Immunofluorescence analysis of methanol-fixed HeLa, using PAPSS1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PAPSS1 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | PAPSS1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate synthase 1)Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAPSS1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Rabbit, Bovine, Guinea pigRat (98%), Zebrafish (90%), Xenopus laevis (95%), Rabbit (97%), Bovine (98%), Guinea pig (96%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATPSK1 antibody PAPSS antibody SK1 antibody PAPSS1 antibody PAPSS 1 antibody PAPS synthase 1 antibody 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1 antibody bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 antibody SK 1 an |
|---|---|
| Molecular Weight(MW) | 71 kDa (note) |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 7.5% SDS PAGE diluted at 1:500
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PAPSS1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using PAPSS1 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
