Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PAPSS2 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded H3347 xenograft, using PAPSS2 antibody at 1:500 dilution.Application
PAPSS2 antibody [C1C3] detects PAPSS2 protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. PAPSS2 antibody [C1C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Product Name | PAPSS2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAPSS2 (3'-phosphoadenosine 5'-phosphosulfate synthase 2)Sulfation is a common modification of endogenous (lipids, proteins, and carbohydrates) and exogenous (xenobiotics and drugs) compounds. In mammals, the sulfate source is 3'-phosphoadenosine 5'-phosphosulfate (PAPS), created from ATP and inorganic sulfate. Two different tissue isoforms encoded by different genes synthesize PAPS. This gene encodes one of the two PAPS synthetases. Defects in this gene cause the Pakistani type of spondyloepimetaphyseal dysplasia. Two alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAPSS2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Rabbit, Rhesus Monkey, Bovine, Guinea pigMouse (96%), Xenopus laevis (88%), Rabbit (98%), Rhesus Monkey (100%), Bovine (95%), Guinea pig (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ATPSK2 antibody SK2 antibody PAPSS2 antibody SK 2 antibody bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 antibody PAPS synthase 2 antibody PAPSS 2 antibody PAPS synthetase 2 antibody bifunctional 3'-phosphoadenosine 5'-phosphosulfat |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (2) |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using PAPSS2 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded H3347 xenograft, using PAPSS2 antibody at 1:500 dilution.
Application
PAPSS2 antibody [C1C3] detects PAPSS2 protein at cytoplasm in rat colon by immunohistochemical analysis. Sample: Paraffin-embedded rat colon. PAPSS2 antibody [C1C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
