Application
Western blot analysis of PARC in Daudi lysate with PARC antibody at (A) 1 and (B) 2 ug/ml.Application
Immunocytochemistry of Parc in Daudi cells with Parc antibody at 1 ug/ml.Application
Immunofluorescence of Bcl-G in Daudi cells with Bcl-G antibody at 2 μg/mL.| Product Name | PARC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PARCThe continued localization of p53 to the nucleus is essential for its function as a tumor suppressor. PARC, a large, Parkin-like ubiquitin ligase has recently been identified as a cytoplasmic anchor protein in p53-associated protein complexes. In the absence of stress, PARC inactivation results in nuclear localization of p53 and activation of p53-dependent apoptosis, while overexpression of this protein promoted cytoplasmic sequestration of p53. Surprisingly, PARC knockout mice were viable and exhibited no obvious phenotype, suggesting that other proteins, such as the highly related cullin family of E3 ubiquitin ligases, may perform similar functions in the absence of PARC. Additionally, it has been suggested that p53 binding to PARC may serve to control PARC function. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PARC p53-associated parkin-like cytoplasmic protein p53associated parkinlike cytoplasmic protein UbcH7associated protein1 UbcH7associated protein 1 UbcH7-associated protein 1 UbcH7 associated protein 1 |
|---|---|
| Target | PARC |
| Tissue Specificity | This antibody is specific for PARC |
Application
Western blot analysis of PARC in Daudi lysate with PARC antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunocytochemistry of Parc in Daudi cells with Parc antibody at 1 ug/ml.
Application
Immunofluorescence of Bcl-G in Daudi cells with Bcl-G antibody at 2 μg/mL.| Positive Control | Blocking Peptide: Cat.No. GTX85085-PEP - PARC Peptide Lysates: Cat. No. GTX23951 - Daudi Cell Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Antibody is supplied in PBS containing 0.02% sodium azide |
