Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARG antibody diluted at 1:1000.| Product Name | PARG antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PARG (poly (ADP-ribose) glycohydrolase)Poly(ADP-ribose) glycohydrolase (PARG) is the major enzyme responsible for the catabolism of poly(ADP-ribose), a reversible covalent-modifier of chromosomal proteins. The protein is found in many tissues and may be subject to proteolysis generating smaller, active products. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human PARG. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PARG99 poly(ADP-ribose) glycohydrolase |
|---|---|
| Target | PARG |
| Molecular Weight(MW) | 111 kDa (note) (4) |
| Cellular Localization | Isoform 1: Nucleus , Isoform 2: Cytoplasm , Isoform 3: Cytoplasm |
Application
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARG antibody diluted at 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
