Application
Immunocytochemistry/Immunofluorescence: Park7(DJ-1) Antibody [ - Park7(DJ-1) detected in human cortex.Application
Western Blot: Park7(DJ-1) Antibody [ - Human HEK293 cells and mouse brain lysates.Application
Western Blot: Park7(DJ-1) Antibody [ - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Park7(DJ-1).| Product Name | Park7 / DJ-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to DJ 1DJ-1 is one of several genes thought to cause autosomal-recessive early-onset parkinsonism. |
| Immunogen | A synthetic peptide corresponding to residues between 150-189 of human DJ-1 (the C-terminus). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| GTX30674 may be used for Western blotting where a band is seen at ~25 kDa. The theoretical molecular weight of DJ-1 is ~20 kDa. It has only been tested on transfected lysates, both human and mouse. It is unknown if it will work on endogenous samples. This antibody has also been tested in immunocytochemistry (human paraffin-embedded sections only) and immunoprecipitation. No other applications have been tested.Suggested working dilutions: *Western Blot - 1:2000 Immunocytochemistry - 1:500 (human)Immunoprecipitation - 5 ul/ml (1:200)*The investigator should determine the optimal working dilution for a specific application.: |
|
| Species Reactivity | HumanMouseRatFishHamster |
| Concentration | 1mg/ml |
| Gene Synonyms | FLJ27376 602533 PARK7 11315 DJ1 DJ-1 Q99497 DJ 1 |
|---|---|
| Target | DJ1 |
| Tissue Specificity | This antibody is specific for DJ-1. |
Application
Immunocytochemistry/Immunofluorescence: Park7(DJ-1) Antibody [ - Park7(DJ-1) detected in human cortex.
Application
Western Blot: Park7(DJ-1) Antibody [ - Human HEK293 cells and mouse brain lysates.
Application
Western Blot: Park7(DJ-1) Antibody [ - Cells were transfected with the pCMV6-ENTRY control (lane 1) or pCMV6-ENTRY PARK7 cDNA (lane 2) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Park7(DJ-1).| Application Notes | GTX30674 may be used for Western blotting where a band is seen at ~25 kDa. The theoretical molecular weight of DJ-1 is ~20 kDa. It has only been tested on transfected lysates, both human and mouse. It is unknown if it will work on endogenous samples. This antibody has also been tested in immunocytochemistry (human paraffin-embedded sections only) and immunoprecipitation. No other applications have been tested.Suggested working dilutions: *Western Blot - 1:2000 Immunocytochemistry - 1:500 (human)Immunoprecipitation - 5 ul/ml (1:200)*The investigator should determine the optimal working dilution for a specific application.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4ºC for short-term storage. Aliquot and store at -20°C for long-term. Avoid repeated freezing and thawing. |
| Storage Buffer | 0.1 ml of whole rabbit antisera. Contains no preservative. |
