Application
Western Blot: Parkin (phospho Ser378) Antibody [ - HEK293 cells transfected with Parkin WT (Phospho) and Parkin S378 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S378 mutant.| Product Name | Parkin (phospho Ser101) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Parkin (phospho-Ser101)Parkin is an E3 ligase in the ubiquitin-proteasome system. Hereditary Parkinson's disease is most commonly caused by mutations in the parkin gene and is characterized by the progressive loss of dopaminergic neurons and the presence of Lewy bodies in the substania nigra (Jenner aet al.,1992). Recent evidence suggests that phosphorylation of parkin at Ser378 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005). |
| Immunogen | Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser378 of human parkin. |
| Modification | p-Ser101 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| We recommend the following starting dilutions: Western Blot 1:1000. Optimal concentration should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Parkin (phospho S101) Parkin (phospho Ser101) Parkin (pS101) Parkin (pSer101) Parkin phospho S101 Parkin phospho Ser101 Phospho Parkin (S101) Phospho Parkin (Ser101) Phospho Parkin (pS101) Phospho Parkin (pSer101) |
|---|---|
| Target | Parkin (phospho-Ser101) |
| Molecular Weight(MW) | 52 kDa (note) |
| Tissue Specificity | Specific for the approx. 52k parkin protein phosphorylated at Ser378. Immunolabeling of the parkin band is absent in parkin S378 mutants. |
Application
Western Blot: Parkin (phospho Ser378) Antibody [ - HEK293 cells transfected with Parkin WT (Phospho) and Parkin S378 mutant (non-phospho) showing the phospho-specific immunolabeling of the ~ 52 k parkin protein. The immunolabeling is absent in the parkin S378 mutant.| Application Notes | We recommend the following starting dilutions: Western Blot 1:1000. Optimal concentration should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.1 ml in 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg per ml BSA and 50% glycerol. |
