Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] diluted at 1:1000.Application
PARP1 antibody [GT982] detects PARP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 protein stained by PARP1 antibody [GT982] diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.Application
Immunoprecipitation of PARP protein from 293T whole cell extracts using 5 μg of PARP antibody . Western blot analysis was performed using PARP antibody . EasyBlot anti-Mouse IgG was used as a secondary reagent.Application
PARP1 antibody detects PARP1 protein by western blot analysis. Un-treated (-) and treated (+, 30uM cisplatin treatment for 24hr) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody at a dilution of 1:1000.| Product Name | PARP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT982] to PARP1 (poly (ADP-ribose) polymerase 1)This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PARP1. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT982 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (87%), Rat (85%), Bovine (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ADPRT 1 poly (ADP-ribose) polymerase 1 PARP ADPRT1 ARTD1 PARP-1 ADPRT PPOL pADPRT-1 |
|---|---|
| Target | PARP1 |
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [GT982] diluted at 1:1000.
Application
PARP1 antibody [GT982] detects PARP1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PARP1 protein stained by PARP1 antibody [GT982] diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.
Application
Immunoprecipitation of PARP protein from 293T whole cell extracts using 5 μg of PARP antibody . Western blot analysis was performed using PARP antibody . EasyBlot anti-Mouse IgG was used as a secondary reagent.
Application
PARP1 antibody detects PARP1 protein by western blot analysis. Un-treated (-) and treated (+, 30uM cisplatin treatment for 24hr) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP1 antibody at a dilution of 1:1000.| Positive Control | 293T , HeLa , HeLa (30uM cisplatin treatment for 24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
