Application
PARP14 antibody detects PARP14 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP14 antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of PARP14 protein from SK-N-SH whole cell extracts using 5 μg of PARP14 antibody . Western blot analysis was performed using PARP14 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | PARP14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PARP14 (poly (ADP-ribose) polymerase family, member 14)Poly(ADP-ribosyl)ation is an immediate DNA damage-dependent posttranslational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP14 belongs to the superfamily of enzymes that perform this modification (Ame et al., 2004 [PubMed 15273990]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PARP14. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BAL2 poly (ADP-ribose) polymerase family member 14 ARTD8 pART8 PARP-14 |
|---|---|
| Target | PARP14 |
| Molecular Weight(MW) | 203 kDa (note) |
| Cellular Localization | Nucleus , Cytoplasm |
Application
PARP14 antibody detects PARP14 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with PARP14 antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of PARP14 protein from SK-N-SH whole cell extracts using 5 μg of PARP14 antibody . Western blot analysis was performed using PARP14 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | U87-MG , SK-N-SH , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
