Application
PARP3 antibody detects PARP3 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE PARP3 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: mouse heart 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PARP3 antibody detects PARP3 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE PARP3 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PARP3 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using PARP3 antibody at 1:100 dilution.| Product Name | PARP3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PARP3 (poly (ADP-ribose) polymerase family, member 3)The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PARP3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ADPRT3 antibody ADPRTL2 antibody ADPRTL3 antibody IRT1 antibody PADPRT-3 antibody PARP3 antibody ADP-ribosyltransferase (NAD+ poly (ADP-ribose) polymerase)-like 3 antibody NAD(+) ADP-ribosyltransferase 3 antibody poly[ADP-ribose] synthase 3 antib |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (2) |
| Cellular Localization | Nucleus , Centrosome , centriole |
Application
PARP3 antibody detects PARP3 protein by western blot analysis. A. 30 µg A431 whole cell lysate/extract B. 30 µg HeLa whole cell lysate/extract C. 30 µg A375 whole cell lysate/extract 7.5% SDS-PAGE PARP3 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: mouse heart 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PARP3 antibody detects PARP3 protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5% SDS-PAGE PARP3 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PARP3 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using PARP3 antibody at 1:100 dilution.| Positive Control | A431 , HeLa , A375 , mouse heart , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
