Application
Pax2a antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Green: Pax2a antibody dilution: 1:200. Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] dilution: 1:50.Application
Pax2a antibody detects Pax2a protein on Medaka by whole mount immunohistochemical analysis. Sample: 7 days-post-fertilization medaka embryo. Pax2a antibody dilution: 1:100.Application
Zebrafish tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pax2a antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Pax2a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Pax2a (paired box gene 2a)Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of zebrafish Pax2a. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHC-W |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Zebra FishMedaka |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | paired box protein Pax-2a antibody paired box gene 2.1 antibody no isthmus protein antibody paired box homeotic gene 2a antibody PAXZF-B antibody Pax-2 antibody cb378 antibody noi antibody pax-b antibody pax2.1 antibody pax2a1 antibody pax[zf-b] |
|---|---|
| Molecular Weight(MW) | 44 kDa (note) |
| Cellular Localization | Nucleus |
Application
Pax2a antibody and Tubulin (Acetyl Lys40) antibody label central nervous system on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Green: Pax2a antibody dilution: 1:200. Red: Tubulin (Acetyl Lys40) antibody [6-11B-1] dilution: 1:50.
Application
Pax2a antibody detects Pax2a protein on Medaka by whole mount immunohistochemical analysis. Sample: 7 days-post-fertilization medaka embryo. Pax2a antibody dilution: 1:100.
Application
Zebrafish tissue extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pax2a antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 36 hpf zebrafish stage |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
