Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAX3 antibody at 1:200 dilution.Application
U87-MG whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SG xenograft, using PAX3 antibody at 1:100 dilution.| Product Name | PAX3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAX3 (paired box 3)This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PAX3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CDHS antibody HUP2 antibody MGC120381 antibody MGC120382 antibody MGC120383 antibody MGC120384 antibody MGC134778 antibody WS1 antibody WS3 antibody PAX3 antibody paired box protein Pax-3 antibody paired box homeotic gene 3 antibody paired doma |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) |
| Tissue Specificity | This antibody is raised against PAX3E isoform. The cross reactivity with other PAX3 isoforms has not been tested. |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PAX3 antibody at 1:200 dilution.
Application
U87-MG whole cell and nuclear extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SG xenograft, using PAX3 antibody at 1:100 dilution.| Positive Control | A431 , H1299 , HeLa , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
