Application
PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of PAX8 antibody The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.Application
PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 μg of PAX8 antibody 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8 antibody at 1:200 dilution.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PAX8 antibody detects PAX8 protein at cytosol and nucleus on rat thyroid gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thyroid gland. PAX8 antibody dilution: 1:500.Application
PAX8 antibody detects PAX8 protein at nucleolus by immunohistochemical analysis. Sample: Paraffin-embedded DLD1 xenograft . PAX8 antibody diluted at 1:500.Application
PAX8 antibody detects PAX8 protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PAX8 antibody diluted at 1:500.| Product Name | PAX8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PAX8 (paired box 8)This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, DogXenopus laevis (91%), Dog (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PAX8 antibody paired domain gene 8 antibody paired box protein Pax-8 antibody paired box 8 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
PAX8 antibody immunoprecipitates PAX8 protein-DNA in ChIP experiments. ChIP Sample: 293T whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of PAX8 antibody The precipitated DNA was detected by PCR with primer set targeting to E2F1 promoter.
Application
PAX8 antibody immunoprecipitates PAX8 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of PAX8 protein by 2 μg of PAX8 antibody 10% SDS-PAGE The immunoprecipitated PAX8 protein was detected by PAX8 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8 antibody at 1:200 dilution.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PAX8 antibody detects PAX8 protein at cytosol and nucleus on rat thyroid gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thyroid gland. PAX8 antibody dilution: 1:500.
Application
PAX8 antibody detects PAX8 protein at nucleolus by immunohistochemical analysis. Sample: Paraffin-embedded DLD1 xenograft . PAX8 antibody diluted at 1:500.
Application
PAX8 antibody detects PAX8 protein at nucleus in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. PAX8 antibody diluted at 1:500.| Positive Control | 293T , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
