Application
WB analysis of SKOV-3, COS7 and HeLa lysates (60ug per lane) using PAX8 antibody [1F8-3A8] at a dilution of 1:1000.Application
IHC-P analysis of human thyroid tissue with (right) or without (left) PAX8 antibody [1F8-3A8] at a dilution of 1:100. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.Application
ICC/IF analysis of 293T (left) and COS7 cells (right) using PAX8 antibody [1F8-3A8] at a dilution of 1:50 (green). F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342.Application
IHC-P analysis of mouse kidney tissue with (right) or without (left) PAX8 antibody [1F8-3A8] at a dilution of 1:20. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.Application
WB analysis of various cell lysates (20ug per lane) using PAX8 antibody [1F8-3A8] at a dilution of 1:500.Application
IHC-P analysis of human kidney tissue with (right) or without (left) PAX8 antibody [1F8-3A8] at a dilution of 1:100. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.Application
WB analysis of lysates from HEK293T cells overexpressing PAX8 (right) or empty vector control (left) PAX8 antibody [1F8-3A8] at a dilution of 1:1000.| Product Name | PAX8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1F8-3A8] to PAX8This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010] |
| Immunogen | Purified internal fragment of human recombinant PAX8 expressed in E. coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F8-3A8 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| IF: 1:50 - 1:200. IHC-P: 1:20 - 1:200. WB: 1:500 - 1:2000: |
|
| Species Reactivity | HumanPrimate |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | paired box 8 |
|---|---|
| Target | PAX8 |
Application
WB analysis of SKOV-3, COS7 and HeLa lysates (60ug per lane) using PAX8 antibody [1F8-3A8] at a dilution of 1:1000.
Application
IHC-P analysis of human thyroid tissue with (right) or without (left) PAX8 antibody [1F8-3A8] at a dilution of 1:100. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Application
ICC/IF analysis of 293T (left) and COS7 cells (right) using PAX8 antibody [1F8-3A8] at a dilution of 1:50 (green). F-Actin (red) was stained with Phalloidin and nuclei (blue) were stained with Hoechst 33342.
Application
IHC-P analysis of mouse kidney tissue with (right) or without (left) PAX8 antibody [1F8-3A8] at a dilution of 1:20. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Application
WB analysis of various cell lysates (20ug per lane) using PAX8 antibody [1F8-3A8] at a dilution of 1:500.
Application
IHC-P analysis of human kidney tissue with (right) or without (left) PAX8 antibody [1F8-3A8] at a dilution of 1:100. To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min.
Application
WB analysis of lysates from HEK293T cells overexpressing PAX8 (right) or empty vector control (left) PAX8 antibody [1F8-3A8] at a dilution of 1:1000.| Application Notes | IF: 1:50 - 1:200. IHC-P: 1:20 - 1:200. WB: 1:500 - 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20°C |
| Storage Buffer | PBS with 1mg/ml BSA, 30% glycerol and 0.05% sodium azide |
