Application
Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody diluted at 1:3000.| Product Name | Paxillin (phospho Ser273) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Paxillin (phospho Ser273) (paxillin) |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Paxillin (phospho Ser273). The exact sequence is proprietary. |
| Modification | p-Ser273 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, ChickenMouse (100%), Rat (100%), Xenopus laevis (100%), Chicken (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | Paxillin (phospho Ser273) |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (4) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell junction , focal adhesion |
Application
Untreated (–) and treated (+) A431 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin (phospho Ser273) antibody diluted at 1:3000.| Positive Control | A431(100 nM Calyculin A treatment for 30 min) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
