Application
Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody diluted at 1:100. Red: phalloidin staining. Courtesy of a researcher who prefers to be anonymous.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Paxillin antibody immunoprecipitates Paxillin protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 2 μg of preimmune Rabbit IgG B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody 7.5 % SDS-PAGE The immunoprecipitated Paxillin protein was detected by Paxillin antibody diluted at 1:10000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Paxillin antibody detects PXN protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. Paxillin antibody dilution: 1:500.Application
Paxillin antibody detects Paxillin protein by western blot analysis. A. 1 µg 293T whole cell extract B. 1 µg whole cell extract of GFP-human Paxillin-transfected 293T cells 7.5% SDS-PAGE Paxillin antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Paxillin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Paxillin (paxillin) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Paxillin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | ChickenChicken (83%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ16691 antibody PXN antibody paxillin antibody |
|---|---|
| Molecular Weight(MW) | 65 kDa (note) (4) |
| Cellular Localization | Cytoplasm , cytoskeleton , Cell junction , focal adhesion |
Application
Paxillin antibody detects Paxillin protein at cytoskeleton by immunofluorescent analysis. Sample: MDA-MB-231 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Paxillin protein stained by Paxillin antibody diluted at 1:100. Red: phalloidin staining. Courtesy of a researcher who prefers to be anonymous.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody diluted at 1:6000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Paxillin antibody immunoprecipitates Paxillin protein in IP experiments. IP samples: HeLa whole cell extract A. Control with 2 μg of preimmune Rabbit IgG B. Immunoprecipitation of Paxillin protein by 2 μg Paxillin antibody 7.5 % SDS-PAGE The immunoprecipitated Paxillin protein was detected by Paxillin antibody diluted at 1:10000. [EasyBlot anti-rabbit IgG was used as a secondary reagent]
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Paxillin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Paxillin antibody detects PXN protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. Paxillin antibody dilution: 1:500.
Application
Paxillin antibody detects Paxillin protein by western blot analysis. A. 1 µg 293T whole cell extract B. 1 µg whole cell extract of GFP-human Paxillin-transfected 293T cells 7.5% SDS-PAGE Paxillin antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | K562 , THP-1 , GFP-human Paxillin-transfected 293T , 293T , A431 , HeLa , HepG2 , A375 , *MDA-MB-231 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
