Application
PBK antibody detects PBK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of PBK protein from A431 whole cell extracts using 5 μg of PBK antibody [N2C3] . Western blot analysis was performed using PBK antibody [N2C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | PBK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PBK (PDZ binding kinase)This genes encodes a serine/threonine kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. This mitotic kinase may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PDZ binding kinase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Chimpanzee, BovineMouse (89%), Rat (91%), Pig (93%), Chimpanzee (99%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CT84 antibody FLJ14385 antibody Nori-3 antibody SPK antibody TOPK antibody PBK antibody MAPKK-like protein kinase antibody T-LAK cell-originated protein kinase antibody cancer/testis antigen 84 antibody lymphokine-activated killer T-cell-originate |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
Application
PBK antibody detects PBK protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBK antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of PBK protein from A431 whole cell extracts using 5 μg of PBK antibody [N2C3] . Western blot analysis was performed using PBK antibody [N2C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | A431 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
