Application
PBR antibody detects PBR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PBR antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of PBR protein from A431 whole cell extracts using 5 μg of PBR antibody . Western blot analysis was performed using PBR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | PBR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PBRPresent mainly in the mitochondrial compartment of peripheral tissues, the protein encoded by this gene interacts with some benzodiazepines and has different affinities than its endogenous counterpart. The protein is a key factor in the flow of cholesterol into mitochondria to permit the initiation of steroid hormone synthesis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PBR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MBR pk18 mDRC DBI PKBS PTBR PBS IBP BZRP translocator protein (18kDa) BPBS TSPO |
|---|---|
| Target | PBR |
| Molecular Weight(MW) | 19 kDa (note) (3) |
| Cellular Localization | Mitochondrion membrane; Multi-pass membrane protein |
Application
PBR antibody detects PBR protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with PBR antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of PBR protein from A431 whole cell extracts using 5 μg of PBR antibody . Western blot analysis was performed using PBR antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | U87-MG , A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
